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Journal of Diabetes Research
Volume 2016, Article ID 5807876, 8 pages
Research Article

In Vitro Proliferation of Porcine Pancreatic Islet Cells for β-Cell Therapy Applications

1Wake Forest Institute for Regenerative Medicine, Wake Forest Baptist Medical Center, Medical Center Boulevard, Winston-Salem, NC 27157, USA
2Virginia Tech, Wake Forest University School of Biomedical Engineering and Sciences, 320 ICTAS, Stanger St., Virginia Tech, Blacksburg, VA 24060, USA

Received 19 August 2016; Revised 4 November 2016; Accepted 13 November 2016

Academic Editor: Hiroshi Okamoto

Copyright © 2016 Guoguang Niu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Supplemental figure 1 shows Islet-derived cell shape in culture. Images of Islet-derived cells were obtained at the indicated passage numbers and the duration (days) after each passage. The cells were cultured in CMRL media containing with 10% FBS (a) or 20% porcine serum (c) and in DMEM media containing 10%FBS (b) with 20% porcine serum (d). Cell size and morphology changed from small-size cobblestone-like shape into big-size fibroblast-like cells during the 5 passages. Supplemental figure 2 Expression of insulin and PDX-1 mRNA expression in islet-derived cells cultured with different media and normalized for 18sRNA expression. Islet derived cells were cultured in media as described in Fig 3A. Analysis of insulin (A) and Pdx1 (B) mRNA expression using qRT-PCR was performed on cells isolated from different pigs and representative results were presented. The results are the average fold increase for each indicated mRNA compared with the level of this mRNA in adipose-derived stem cells (ADSC), and normalized for the expression of 18sRNA.

  1. Supplementary Material