Diabetes Mellitus and Increased Tuberculosis Susceptibility: The Role of Short-Chain Fatty Acids

Figure 2

Influence of butyrate on HDAC expression and activity. (a) PBMCs were preincubated with 50 μM C4 for 1 h prior to stimulation with Mtb lysate or LPS for 4 h. Gene expression levels of HDAC1 and HDAC8 were measured by qPCR. Data are means ± SEM (), using Wilcoxon signed-rank test, representative of 3 independent experiments. . (b) Percentage of general HDAC activity relative to RPMI stimulated PBMCs, as measured by levels of substrate deacetylation after 30 min of preincubation with C4 (50 μM) and 30 min of stimulation with Mtb lysate. Data are means ± SEM ( to 6), using Wilcoxon signed-rank test, representative of 3 independent experiments. .

(a)

(b)