Noninvasive Tracking of Encapsulated Insulin Producing Cells Labelled with Magnetic Microspheres by Magnetic Resonance Imaging

Figure 2

Viability and insulin secretion of nonencapsulated MM-labelled cells. (a) Viability images of unlabelled and MM-labelled MIN6 (i and iii) and human islets (ii and iv), respectively. Bar is 50 μm for (i) and (iii) and 100 μm for (ii) and (iv). Viability is estimated as a percentage of green (live cells) to red (dead cells) fluorescence. Values are mean ± SEM ( aliquots for MIN6 and clusters for islets). (b) Glucose stimulated insulin secretion of both MIN6 (i) and human islets (ii) 24 hr after MM labelling. Values are mean ± SEM (); and for basal versus stimulated insulin secretion for both unlabelled and MM-labelled MIN6 and human islets, respectively (Student’s t-test).

(a)

(b)