Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 2

Overexpression of Nr4a1 and Nr4a3 induces Cdk5r1 expression in primary rat islets. Rat islets were treated with AdCMV-GFP, AdCMV-Nkx6.1, AdCMV-Nr4a1, or AdCMV-Nr4a3. (a) Cdk5r1 mRNA levels increase is induced by Nr4a1 and Nr4a3 but not by Nkx6.1, while (b) Cdk5 mRNA levels are unchanged by overexpression of Nkx6.1, Nr4a1, or Nr4a3. Data represent the of six independent experiments. (c) Representative western blot and quantitation demonstrate that islets transduced with AdCMV-Nr4a1 or AdCMV-Nr4a3 induce upregulation of Cdk5r1 protein levels. Data represent the of six independent experiments. (d) Representative western blot and quantitation demonstrate that islets transduced with AdCMV-Nr4a1 and AdCMV-Nr4a3 have no additive increase on Cdk5r1 protein levels. Data represent the of three experiments for western blots. ; . p value represents the comparison between Nr4a1- or Nr4a3-treated and GFP-treated islets.

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