Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 3

Overexpression of Cdk5r1 is sufficient to induce primary rat islet proliferation. (a) Islets were transduced with AdCMV-GFP or AdCMV-Cdk5r1. Protein was harvested 96 hours after viral transduction. A 6-fold increase was observed in Cdk5r1 protein levels in islets transduced with AdCMV-Cdk5r1, as compared to the observed low endogenous level in primary rat islets. Data represent the of six independent experiments representing the comparison between untreated islets and islets transduced with AdCMV-Cdk5r1. (b) Incorporation of [³H-methyl]-thymidine in rat islets. Rat islets transduced with AdCMV-Cdk5r1 have increased proliferation, while islets treated with AdCMV-GFP or AdCMV-Cdk5 have no induction of proliferation. Data represent the of four independent experiments representing the comparison between untreated islets and islets transduced with AdCMV-Cdk5r1. (c) Islets were transduced with AdCMV-GFP, Cdk5r1, or Nkx6.1 or were transduced with AdCMV-Nkx6.1 and either GFP or Cdk5r1. Islets were labeled with ³H-thymidine 72 hours after viral transduction, followed by measurements of proliferation at 96 hours after viral transduction. Data represent the of four independent experiments representing the comparison between AdCMV-Nkx6.1 treated islets and islets cotransduced with AdCMV-Nkx6.1 and AdCMV-Cdk5r1. ; .

(a)

(b)

(c)