Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 4

Overexpression of Cdk5r1 is sufficient to induce β-cell proliferation. (a) Percentage of BrdU+Insulin+ islet cells cultured with BrdU for 96 hours. (b) Representative image (40x magnification) of dispersed rat islets transduced with AdCMV-GFP labeled with BrdU. (c) Representative image (40x magnification) of dispersed rat islets transduced with AdCMV-Cdk5r1 labeled with BrdU. Arrows indicate BrdU+Insulin+ nuclei. DAPI is blue, insulin is green, and BrdU is red. Data represent the of four independent experiments. . p value represents the comparison between Cdk5r1- and GFP-treated islets.

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