Cdk5r1 Overexpression Induces Primary β-Cell Proliferation

Figure 8

Overexpression of Cdk5r1 results in increased pRb phosphorylation. (a) Untransduced islets were compared to islets transduced with AdCMV-GFP, AdCMV-Cdk5, or AdCMV-Cdk5r1, and levels of phosphorylated pRb were measured. Representative western blot from four independent experiments. (b) Untreated islets and islets transduced with AdCMV-GFP, AdCMV-Cdk5, or AdCMV-Cdk5r1 were treated with 10 μM roscovitine and levels of phosphorylated pRb were measured. Representative western blots and quantitation from three independent experiments. ; ; .

(a)

(b)