Altered Macrophage and Dendritic Cell Response in Mif−/− Mice Reveals a Role of Mif for Inflammatory-Th1 Response in Type 1 Diabetes

Figure 9

Macrophages and monocytes from Mif−/− mice have impaired ability to induce lymphocyte proliferation. At 8 weeks after STZ treatment, Wt (■) or Mif−/− (□) Mφ (F4/80⁺) and monocytes (CD11b⁺) primed with 10 μg/mL OVA were cocultured with OVA-transgenic T cells for 5 days. Subsequently, [3H]-thymidine was added for 18 h, and [3H]-thymidine incorporation was measured. The values are presented as means ± SEM counts per minute (CPM) from triplicate wells of three independent experiments (). or . GraphPad Prism software 6, GraphPad Prism software 6.

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