Hypoxia Alters the Expression of Dipeptidyl Peptidase 4 and Induces Developmental Remodeling of Human Preadipocytes

Figure 2

Oxygenation-dependent expression density and shedding of DPP4 in human preadipocytes cultured in vitro. (a) Confocal images (upper panels) and transmission light microscopy images (lower panels) of human preadipocytes (subconfluent cultures) immunolabeled with antibody against DPP4. Each panel shows representative confocal images of a cell incubated for 2 days (A, B, E, and F) and 9 days (C, D, G, and H) under normoxic (A, C, E, and G) and hypoxic (B, D, F, and H) environmental conditions. Scale bars indicate 10 μm. (b) Relative expression density of DPP4 in human preadipocytes cultured for 2 and 9 days in normoxic or hypoxic conditions. Numbers denote the number of cells imaged. (c) Normalized DPP4 protease activity in conditioned medium. Asterisks denote statistically significant difference (; ; , two-way ANOVA).

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