Metformin Treatment Prevents Sedentariness Related Damages in Mice

Figure 4

METF action on C2C12 differentiation and neoformed myotubes. (a) Representative Western blots for C2C12 cell lysates and the indicated antibodies. (b) METF enhanced myotube formation rising MyHC, Myogenin, and N-cadherin protein levels, improved mitochondrial antioxidant proprieties SOD2, activated ERK, and deactivated p70 signaling pathways. (c) Phase contrast images showed that differentiation efficacy is optimal in our model, since all competent myocytes became multinucleated myotubes. (d) At 48 h from differentiation induction, immunofluorescence analysis indicated positive role of METF in CAMKII protein. (e) METF action on neoformed myotubes: METF improved MyHC protein content and activated ERKs. (f) Immunofluorescence studies confirmed Western blot data of METF action in hypertrophic process. Scale bar 200 μm. Significance: .

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