Hyperglycemia Does Not Affect Iron Mediated Toxicity of Cultured Endothelial and Renal Tubular Epithelial Cells: Influence of L-Carnosine

Figure 2

Influence of high glucose and carnosine on iron mediated toxicity in HUVECs (a, c, and e) and PTECs (b, d, and f). High glucose concentration neither increased cell susceptibility to iron nor disrupted the protective effect of carnosine on iron toxicity in HUVECs (a) and PTECs (b). The protective effect of carnosine showed a clear dose-dependent relation in both HUVECs (c) and PTECs (d). Carnosine was only protective when presented during iron challenge but not when cells were pretreated (e and f). Like carnosine also the iron chelator DFO prevented iron mediated toxicity in HUVEC when iron concentration was less than 600 μM (g). Med: medium, CN: carnosine, Glc: high glucose, and Glc + CN: high glucose + carnosine.

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