Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy

Figure 2

Effects of NAR on cell proliferation, cell cycle, and deposition of ECM proteins. (a) MTT analyses of cell proliferation rates. (b) Quantifications of the phases of the cell cycle analysis by flow cytometry. (c) Real-time RT PCR analyses of Col4 and FN mRNAs from kidney tissues. (d) Western blot analysis of Col4 and FN proteins in kidney tissues and MMCs. (e) Quantifications of the western blot bands of Col4 and FN expressions in DN rats. (f) Quantifications of the western blot bands of Col4 and FN expressions in MMCs. (g) Immunofluorescence of Col4 and FN proteins in kidney tissues of rats. (h) Immunofluorescence of Col4 and FN proteins in MMCs. (a, b) Results are expressed as mean ± standard deviation ( per group). ; versus HG group. (c) Results were normalized with GAPDH and expressed as mean ± standard deviation ( per group). versus CON group; versus DN group. (e) Results were normalized with GAPDH and expressed as mean ± standard deviation ( per group). ; versus CON group; versus DN group. (f) Results were normalized with GAPDH and expressed as mean ± standard deviation ( per group). ; versus LG group; ; versus HG group.

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