Naringenin Ameliorated Kidney Injury through Let-7a/TGFBR1 Signaling in Diabetic Nephropathy

Figure 4

TGFBR1 was a target for let-7a. (a) Let-7a binding region of the 3′-UTR of TGFBR1 from fifteen species. Paired bases were exhibited by a black line. And the binding site was boxed with a black rectangle. (b) Luciferase activity of reporters in the presence or absence of let-7a mimics in 293T cells. Luc activity was significantly decreased in TGFBR1-wt+let7a-M. (c) Let-7a negatively regulated the expression of TGFBR1 in MMCs by western blot. (d) Quantifications of the western blot bands. (b) Data shown as mean ± standard deviation ( per group). versus TGFBR1-wt+NC-M group. (d) Results were normalized with GAPDH and expressed as mean ± standard deviation ( per group). versus LG group; versus HG group.

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