High-Fat Diet Augments VPAC1 Receptor-Mediated PACAP Action on the Liver, Inducing LAR Expression and Insulin Resistance

Figure 2

Insulin-induced Akt phosphorylation in liver. Western blot analysis using anti-phospho Akt and anti-Akt antibodies. Liver samples were collected form wild type (WT) and PACAP null (PACAP^−/−) mice fed regular chow and HFD for 6 weeks. The intensity of Akt phosphorylation is expressed by the ratio of phosphorylated over total Akt proteins. . All data are presented as means ± s.e.m. .