The Yin and Yang of the Opioid Growth Regulatory System: Focus on Diabetes—The Lorenz E. Zimmerman Tribute Lecture

Figure 3

Immunohistochemistry for OGF and OGFr in mouse (a–c), bird (d–f), and turtle (g–i) corneal epithelium using brightfield (a, d, g) or indirect (b, c, e, f, h, i) immunofluorescence. Insets (a, d, g) are stained with hematoxylin and eosin and demonstrate the corneal epithelium (ep) containing basal and suprabasal cells with the underlying stroma (st). Immunofluorescence microscopy of tissue stained with anti--enkephalin (OGF) IgG (b, e, h) demonstrates OGF in the epithelial cortical cytoplasm of basal and suprabasal cells (arrows). Immunofluorescence of tissues stained with OGFr IgG (arrowheads in c, f, i) (derived from [5]).

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