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Journal of Diabetes Research
Volume 2017, Article ID 2180819, 9 pages
https://doi.org/10.1155/2017/2180819
Research Article

Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus

1Department of Diagnosis and Surgery, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, Brazil
2Department of Morphology, School of Dentistry, Universidade Estadual Paulista (UNESP), Araraquara, SP, Brazil
3Postgraduate Program in Sciences of the University of Franca, Franca, SP, Brazil
4Department of Genetics, Faculty of Medicine of Ribeirão Preto and Department of Biology, FFCLRP, University of São Paulo (USP), Ribeirão Preto, SP, Brazil
5Department of Medical Genetics, University of Campinas (UNICAMP), Campinas, SP, Brazil

Correspondence should be addressed to Raquel Mantuaneli Scarel-Caminaga; rb.psenu.raof@leuqar

Received 16 April 2017; Revised 22 May 2017; Accepted 31 May 2017; Published 25 July 2017

Academic Editor: Hiroshi Okamoto

Copyright © 2017 Sâmia Cruz Tfaile Corbi et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Despite increasing research in type 2 diabetes mellitus (T2D), there are few studies showing the impact of the poor glycemic control on biological processes occurring in T2D. In order to identify potential genes related to poorly/well-controlled patients with T2D, our strategy of investigation included a primary screen by microarray (Human Genome U133) in a small group of individuals followed by an independent validation in a greater group using RT-qPCR. Ninety patients were divided as follows: poorly controlled T2D (G1), well-controlled T2D (G2), and normoglycemic individuals (G3). After using affy package in R, differentially expressed genes (DEGs) were prospected as candidate genes potentially relevant for the glycemic control in T2D patients. After validation by RT-qPCR, the obtained DEGs were as follows—G1 + G2 versus G3: HLA-DQA1, SOS1, and BRCA2; G2 versus G1: ENO2, VAMP2, CCND3, CEBPD, LGALS12, AGBL5, MAP2K5, and PPAP2B; G2 versus G3: HLA-DQB1, MCM4, and SEC13; and G1 versus G3: PPIC. This demonstrated a systemic exacerbation of the gene expression related to immune response in T2D patients. Moreover, genes related to lipid metabolisms and DNA replication/repair were influenced by the glycemic control. In conclusion, this study pointed out candidate genes potentially associated with adequate glycemic control in T2D patients, contributing to the knowledge of how the glycemic control could systemically influence gene expression.