Journal of Diabetes Research

Research Article

Expression Profile of Genes Potentially Associated with Adequate Glycemic Control in Patients with Type 2 Diabetes Mellitus

Table 2

DEGs detected by microarray chosen as good candidates for validation by RT-qPCR.


Comparison	Gene title	Gene symbol	Microarray FC (FDR value)	RT-qPCR value (validation)

G1 + G2 versus G3
^@213831_at #Hs03007426 mH	Major histocompatibility complex, class II, DQ alpha 1	HLA-DQA1	127.80 (0.002)	<0.0001
^@212777_at #Hs00893134_m1	Son of sevenless homolog 1 (drosophila)	SOS1	2.60 (0.005)	0.005
^@222381_at #Hs00918237_m1	Aryl-hidrocarbon receptor repressor	PDCD6	−2.43 (0.007)	NV
^@208368_s_at #Hs00609073_m1	Breast cancer 2, early onset	BRCA2	−1.46 (0.009)	0.008
^@210808_s_at #Hs01071081_m1	NADPH oxidase 1	NOX1	−1.43 (0.002)	NV

G2 versus G1
^@231857_s_at #Hs01005454_g1	ATP/GTP-binding protein-like 5	AGBL5	6.08 (0.002)	0.042
^@1562028_at #Hs00236949_m1	Cyclin D3	CCDN3	1.99 (0.006)	0.0003
^@213006_at #Hs00270931_s1	CCAAT/enhancer-binding protein (C/EBP), delta	CEBPD	1.89 (0.007)	<0.0001
^@214792_x_at #Hs00360269_m1	Vesicle-associated membrane protein 2	VAMP2	1.67 (0.001)	0.0001
^@201313_at #Hs01102367_g1	Enolase 2 (gamma, neuronal)	ENO2	1.66 (0.0008)	<0.0001
^@211370_s_at #Hs00177134_m1	Mitogen-activated protein kinase 5	MAP2K5	1.39 (0.001)	0.027
^@212226_s_at #Hs00170359_m1	Phosphatidic acid phosphatase type 2B	PPAP2B	−2.68 (0.005)	0.021
^@223828_s_at #Hs00263821_m1	Lectin, galactoside-binding, soluble 12	LGALS12	−2.09 (0.007)	0.0004

G2 versus G3
^@209480_at #Hs03054971_m1	Major histocompatibility complex, class II, DQ beta 1	HLA-DQB1	131.31 (0.005)	<0.0001
^@211571_s_at #Hs00171642_m1	Versican	VCAN	1.73 (0.009)	NV
^@212142_at #Hs00907398_m1	Minichromosome maintenance	MCM4	−4.46 (0.0001)	0.0006
^@239617_at #Hs01115007_m1	SEC13 homolog (S. cerevisiae)	SEC13	−2.34 (0.007)	0.008
^@205672_at #Hs00166045_m1	Xeroderma pigmentosum, complementation group A	XPA	−1.29 (0.003)	NV

G1 versus G3
^@204517_at #Hs00917412_m1	Peptidylprolyl isomerase C (cyclosphilin C)	PPIC	2.21 (0.008)	0.001
^@1554586_a_at #Hs01598095_g1	Rho-related BTB domain containing 2	RHOBTB2	2.13(0.007)	NV
^@1559921_at #Hs00169777_m1	Platelet/endothelial cell adhesion molecule (CD31 antigen)	PECAM1	1.86 (0.004)	NV
^@215723_s_at #Hs00160118_m1	Phospholipase D1, phosphatidylcholine-specific	PLD1	−3.77 (0.005)	NV

FC = fold change; FDR = false discovery rate method according the Benjamini-Hochberg procedure was used for correcting each value for multiple hypothesis testing; NV = microarray not validated by RT-qPCR; ^@ = probe set ID; # = TaqMan code of Applied Biosystems.