Pirfenidone Accelerates Wound Healing in Chronic Diabetic Foot Ulcers: A Randomized, Double-Blind Controlled Trial
Table 3
Gene Expression.
Gene
Baseline
PFD + M-DDO
KTS
1st month
2nd month
1st month
2nd month
Col-1α
1
49.00 ± 38.06+
69.15 ± 62.02+
1.08 ± 0.48
0.17 ± 0.08
Col-4
1
22.09 ± 9.02+
2.35 ± 0.52
13.59 ± 5.70
1.02 ± 0.62
TGF-β1
1
1.23 ± 0.37
2.99 ± 1.64+
1.97 ± 0.56
0.80 ± 0.26
TGF-β3
1
6.66 ± 4.83
16.62 ± 15.02+
1.50 ± 0.40
0.73 ± 0.33
α-SMA
1
111.41 ± 32.44+
14.53 ± 8.81
2.74 ± 1.34+
3.02 ± 2.77
Elastin
1
356.53 ± 212.17
122.37 ± 77.39
8.29 ± 2.74
13.02 ± 6.09
Fibronectin
1
6.26 ± 2.25
4.39 ± 2.34
0.79 ± 0.23
1.44 ± 0.25
MMP-1
1
2.23 ± 0.95
11.25 ± 9.11
3.38 ± 1.56
1.49 ± 0.72
KGF
1
8.75 ± 5.20+
5.42 ± 3.45+
11.02 ± 5.81
21.59 ± 16.97
HIF-1α
1
1.59 ± 0.51+
1.95 ± 0.82
1.51 ± 0.31
1.09 ± 0.49
HIF-1β
1
1.55 ± 0.49
1.32 ± 0.52
2.95 ± 1.33
1.09 ± 0.29
VEGF
1
1.27 ± 0.37
2.07 ± 1.11
2.27 ± 0.60
0.92 ± 0.42
The table shows gene expression at baseline, first, and second months of treatment. Gene expression was normalized against the housekeeping gene 18S. Relative quantification was performed using the 2−ΔΔCT method. Data are expressed as mean + SEM. +Significant difference with baseline. Significant difference between groups.
