Differentially Expressed MicroRNAs in the Development of Early Diabetic Retinopathy

Figure 4

Quantitative reverse transcription PCR analysis showing SP1 mRNA upregulation in both HRECs and RPE cells exposed to hyperglycemia. SP1 expression in (a) HRECs and (b) RPE cells increased with HG-exposure time. SP1 mRNA levels were normalized relative to the mRNA levels of PUM1 and PPIA in HRECs and RPE cells, respectively. Culturing of HRECs and RPE cells with MN revealed that osmotic change did not affect SP1 expression. ; , compared with NG.

(a)

(b)