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Journal of Diabetes Research
Volume 2017, Article ID 5649191, 8 pages
https://doi.org/10.1155/2017/5649191
Research Article

Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro

1Institute of Tropical Medicine, Third Military Medical University, Chongqing, China
2Bioengineering College, Chongqing University, Chongqing, China
3Xi’an Center for Disease Control and Prevention, Xi’an, China
4Department of Hematology, Southwest Hospital, Chongqing, China
5Biomedical Analysis Center, Third Military Medical University, Chongqing, China
6Department of Plastic and Aesthetic Surgery, Southwest Hospital, Chongqing, China

Correspondence should be addressed to Zhijia Ye; nc.ude.ummt@eyjz

Received 8 January 2017; Accepted 19 April 2017; Published 3 July 2017

Academic Editor: Marco Songini

Copyright © 2017 Yuewen Han et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The objective of this study was to assess the effects of exogenously expressed proinsulin on the biological characters of a hematopoietic stem cell line (HSC) and erythroid myeloid lymphoid (EML) cells and explore new strategies for cell therapy for type I diabetes. EML cells were transduced with lentivirus particles carrying the human proinsulin (proINS) gene. The positive transduced cells were selected based on green fluorescence protein (GFP) positivity and puromycin resistance. Overexpression of proINS was confirmed via real-time PCR and Western blotting. The functional activity of the human proINS secreted by EML cells was elucidated by analyzing the activation of insulin receptor and its downstream signaling. Pro-INS + EML cells were able to prime the phosphorylation of insulin receptor as well as induce the expression of downstream genes of insulin receptor. Furthermore, Wnt3a can significantly promote self-renewal of Pro-INS + EML cells. However, we did not observe significant changes in the proliferation and differentiation of INS + EML cells, compared to the control EML cells. Our results might be useful for developing a new therapy for diabetes mellitus.