Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro

Figure 3

Effects of exogenous expression of proinsulin on the proliferation, self-renewal, and differentiation of EML cells. (a) EML/empty cells and EML/Pro-INS cells were cultured in fresh EML medium at an initial concentration of 1 × 10⁵. Cell numbers were determined using trypan blue on days 1 and 2. (b) The distribution of the CD34⁺Sca-1hi and CD34⁻Sca-1low subpopulations in EML/empty cells and EML/Pro-INS cells was studied via FACS. (c) CFU-based assay of the differentiation capacity of EML/empty cells and EML/Pro-INS cells into erythroid, granulocyte-macrophage, and megakaryocytic lineages. (d) Analysis of the cell cycle profiles of EML/empty cells and EML/Pro-INS cells by propidium iodide staining.

(a)

(b)

(c)

(d)