Proinsulin Promotes Self-Renewal of a Hematopoietic Progenitor Cell Line In Vitro

Figure 4

Synergistic effects of proinsulin and Wnt3a on the proliferation, self-renewal, and differentiation of EML cells. (a) EML/empty cells and EML/Pro-INS cells were cultured in fresh EML medium with or without Wnt3a (50 ng/ml) at an initial concentration of 1 × 10⁵ (day 0). The total number of living cells assessed via trypan blue staining was counted on days 1 and 2. (b) The distribution of CD34⁺Sca-1hi and CD34⁻Sca-1low subpopulations was detected via FACS. (c) The percentage of lineage marks of B220 and CD11b was tested in the above cells via FACS. and .

(a)

(b)

(c)