Glycopatterns of Urinary Protein as New Potential Diagnosis Indicators for Diabetic Nephropathy

Figure 4

Validation of the differential expressions of the glycopatterns in the urine associated with DN. (a) Scan images of Cy3-labeled SNA and STL incubating to urinary protein microarray, respectively. The incubation images were extracted by GenePix 3.0. (b) Scatter plot analysis of the original data obtained from the urinary protein microarrays. The statistical significance of the differences between groups was calculated by Kruskal-Wallis test. (c) ROC curve analysis of STL to differentiate DN and NDRD, AUC = 0.968, and . (d) SDS-PAGE and lectin blot of pooled urinary protein using SNA and STL, M, marker; lane 1, HVs; lane 2, patients with T2DM; lane 3, DN group I; lane 4, DN group II; lane 5, patients with MN; lane 6, patients with IgAN. (e) The lectin binding strength of selected protein bands is displayed, and the binding strength was counted by image J.

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