TY - JOUR A2 - Fiorina, Paolo AU - Pahwa, Roma AU - Balderas, Miriam AU - Jialal, Ishwarlal AU - Chen, Xinpu AU - Luna, Ruth Ann AU - Devaraj, Sridevi PY - 2017 DA - 2017/12/31 TI - Gut Microbiome and Inflammation: A Study of Diabetic Inflammasome-Knockout Mice SP - 6519785 VL - 2017 AB - Aims. Diabetes is a proinflammatory state, evidenced by increased pattern recognition receptors and the inflammasome (NOD-like receptor family pyrin domain (NLRP)) complex. Recent reports have elucidated the role of the gut microbiome in diabetes, but there is limited data on the gut microbiome in NLRP-KO mice and its effect on diabetes-induced inflammation. Methods. Gut microbiome composition and biomarkers of inflammation (IL-18, serum amyloid A) were assessed in streptozotocin- (STZ-) induced diabetic mice on a NLRP3-knockout (KO) background versus wild-type diabetic mice. Results. SAA and IL-18 levels were significantly elevated in diabetic mice (STZ) compared to control (WT) mice, and there was a significant attenuation of inflammation in diabetic NLRP3-KO mice (NLRP3-KO STZ) compared to control mice (p<0.005). Principal coordinate analysis clearly separated controls, STZ, and NLRP3-KO STZ mice. Among the different phyla, there was a significant increase in the Firmicutes : Bacteroidetes ratio in the diabetic group compared to controls. When compared to the WT STZ group, the NLRP3-KO STZ group showed a significant decrease in the Firmicutes : Bacteroidetes ratio. Together, these findings indicate that interaction of the intestinal microbes with the innate immune system is a crucial factor that could modify diabetes and complications. SN - 2314-6745 UR - https://doi.org/10.1155/2017/6519785 DO - 10.1155/2017/6519785 JF - Journal of Diabetes Research PB - Hindawi KW - ER -