Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins

Figure 1

AR inhibition prevents HG-induced Thp1 cell viability. Thp1 cells (3000 cells/well) were pretreated with fidarestat for overnight followed by incubation with HG (25 mM) for another 48 h. (a) Cell viability was determined by MTT assay. (b) Live and dead cell counts were determined by staining with trypan blue using a hemocytometer. (c) AR activity was determined spectrophotometrically using glyceraldehyde as a substrate. Data represent mean ± SD (). when compared with control, and # when compared with the HG-treated group.

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