Aldose Reductase Inhibitor Protects against Hyperglycemic Stress by Activating Nrf2-Dependent Antioxidant Proteins

Figure 3

AR inhibitor induces the expression of Nrf2-dependent antioxidant enzymes in HG-treated Thp1 cells. Thp1 cells were pretreated with fidarestat for 14 h followed by incubation with HG (25 mM) for another 24 h. Equal amounts of proteins were subjected to Western blot analysis for the expression of HO1 and NQO1. Fold changes were determined after normalizing with loading control GAPDH. A representative blot from three independent analyses is shown (a). The HO1 levels in the cell lysates were determined by ELISA (b). The mRNA levels of the HO1 gene in Thp1 cells were determined by RT-PCR as described in Section 2 (c). SOD and catalase activities were analyzed in Thp1 cell lysates by using specific kits as per the manufacturer’s instructions (d and e). Data represent mean ± SD (). versus control; # when compared with the HG-treated group or the Fidarestat alone-treated group.

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