Suppression of Excessive Histone Deacetylases Activity in Diabetic Hearts Attenuates Myocardial Ischemia/Reperfusion Injury via Mitochondria Apoptosis Pathway

Figure 2

Myocardial infarct size and HDAC activity assessment in diabetic and normal rats. (a) Infarct area versus area at risk (IA/AAR × 100%). (b) Effect of MI/R and diabetes per se on total HDAC activity in myocardium. (c, d) Western blot analysis of myocardium acetylated histones H3 and H4 (Ac-H3, Ac-H4), with histones 3 or 4 (H3, H4) serving as the loading control. (e) p-Akt (ser473) and Akt protein expression in myocardium. All the results are presented as mean ± SEM, /group. versus NS group. versus DS group. versus NIR group.

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