Suppression of Excessive Histone Deacetylases Activity in Diabetic Hearts Attenuates Myocardial Ischemia/Reperfusion Injury via Mitochondria Apoptosis Pathway

<div>Effect of HDAC inhibition on mitochondrial membrane potential and integrity of mitochondrial permeability transition pore (mPTP). Representative fluorescent images and quantitative analysis in each group. (a) Red fluorescence represented JC-1 aggregation on the mitochondria membrane, reflecting the mitochondrial membrane potential (<svg height="8.8423pt" id="M73" style="vertical-align:-0.2064009pt" version="1.1" viewbox="-0.0498162 -8.6359 27.3063 8.8423" width="27.3063pt" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink"><g transform="matrix(.013,0,0,-0.013,0,0)"><path d="M600 0V24L339 665L311 657L43 24V0H600ZM497 50H107L303 539L497 50Z" id="g113-133"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="641" vert-adv-y="641"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,7.413,0)"><path d="M699 659C666 654 622 640 595 610C579 591 569 572 562 530L552 472C530 342 504 307 403 302V529C403 604 411 617 483 622V650H238V622C310 617 318 604 318 529V302C232 308 194 347 172 471L161 531C153 573 145 592 131 609C103 643 59 654 25 659L15 632C62 611 63 583 73 524L85 452C97 383 116 347 155 316C199 282 255 269 318 267V122C318 46 310 33 233 28V0H489V28C412 33 403 46 403 122V267C470 269 518 283 559 315C599 346 618 378 632 450L646 519C658 580 665 608 709 632L699 659Z" id="g113-152"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="725" vert-adv-y="725"></glyph.data></g><g transform="matrix(.013,0,0,-0.013,16.843,0)"><path d="M766 88L752 113C719 83 690 64 681 64C674 64 672 74 679 103L724 292C758 436 724 448 701 448C680 448 666 442 639 429C594 407 514 350 441 252H439L447 289C476 423 450 448 419 448C398 448 379 441 355 427C307 400 234 344 162 249H160L180 324C203 409 197 448 170 448C144 448 82 413 23 349L35 321C57 343 96 374 108 374C115 374 117 371 111 341C87 227 57 112 24 -6L32 -12C53 -4 81 4 108 6C119 68 134 128 149 171C177 229 309 383 364 383C387 383 388 355 373 282C354 190 330 92 303 -6L309 -12C332 -4 356 3 386 6C396 63 411 122 424 171C458 236 590 383 642 383C658 383 664 369 652 315L603 91C587 20 593 -12 619 -12C642 -12 708 23 766 88Z" id="g113-110"></path><glyph.data ascent="3473" descent="-2876" horiz-adv-x="789" vert-adv-y="789"></glyph.data></g></svg>). Green emission of the dye represented the monomeric form of JC-1 after mitochondrial membrane depolarization. (b) Fluorescent images of the cells show the change in integrity of mPTP. Reduced fluorescence in cells indicate opening of mPTP. 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Journal of Diabetes Research

fig8

Figure 8

Figure 8: Suppression of Excessive Histone Deacetylases Activity in Diabetic Hearts Attenuates Myocardial Ischemia/Reperfusion Injury via Mitochondria Apoptosis Pathway 

Figure 8 | Suppression of Excessive Histone Deacetylases Activity in Diabetic Hearts Attenuates Myocardial Ischemia/Reperfusion Injury via Mitochondria Apoptosis Pathway 