Reduced Histone H3 Lysine 9 Methylation Contributes to the Pathogenesis of Latent Autoimmune Diabetes in Adults via Regulation of SUV39H2 and KDM4C

Figure 3

The mRNA level of histone methyltransferase and demethylase in CD4⁺ T lymphocytes from LADA patients. Data are normalized against beta-actin. The ΔCt values were from 0.5 to 3.9. (a) Relative mRNA levels of histone methyltransferases (SET1, G9a, SUV39H1, and SUV39H2) in CD4⁺ T lymphocytes from LADA patients () and healthy controls (), as measured by real-time PCR. The absolute of fold change > 2 was considered significant, LADA patients versus healthy controls; data are normalized against beta-actin. (b) Relative mRNA levels of histone demethylases (LSD1, KDM3A, KDM4A, KDM4B, KDM4C, KDM5A, KDM5B, KDM5C, and KDM5D) in CD4⁺ T lymphocytes from LADA patients () and healthy controls (), the absolute of fold change > 2 was considered significant, LADA patients versus healthy controls; data are normalized against beta-actin.

(a)

(b)