Journal of Diabetes Research / 2017 / Article / Fig 2

Research Article

Hmox1 Deficiency Sensitizes Mice to Peroxynitrite Formation and Diabetic Glomerular Microvascular Injuries

Figure 2

Mice with Hmox haploinsufficiency develop glomerular microvascular lesions under diabetes. (a) Representative images of Masson’s trichrome staining (left panel), hematoxylin/eosin staining (middle panel), and nitrotyrosine immunohistochemistry (brown, right panel) in 20-week-old WT diabetic and Hmox+/− diabetic mice. Scale bar 50 μm (left and middle panel) and 100 μm (right panel). (b) qPCR analysis of Tgfb1, Col1a1, Col3a1, and Kim1 mRNA expression in renal cortex extracts from 20-week-old WT diabetic and Hmox+/− diabetic mice. Data represent means ± sem of 5-6 mice. (c) Endothelial cells (CD31, green) and podocyte (nephrin, red) were stained in 20-week-old WT diabetic and Hmox+/− diabetic mice. Scale bar 50 μm. Nuclei were stained with DAPI (blue). (d) Representative photomicrographs of transmission electron microscopy sections of glomerular capillary loops of 20-week-old WT diabetic and Hmox+/− diabetic mice, showing loss of endothelial fenestrations () and GBM inclusions (arrows) in Hmox+/− diabetic mice. Scale bar 1 μm.
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