Research Article

Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

Figure 3

Effects of ACOS on the expression of podocyte-associated molecules in the rat DN model. (a) Total RNA was extracted from renal cortex tissue, and the relative mRNA expression levels of nephrin, podocin, WT-1, and desmin were measured by real-time quantitative PCR. (b) Renal cortex tissue was lysed, and total lysates were analyzed by the Western blot assay with antibodies against nephrin, podocin, WT-1, desmin, and β-actin, respectively. The relative protein expression level was expressed as the target protein/β-actin ratio. Values are represented as mean ± SD. and vs. control group, and vs. DN model group.
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