Artificially Cultivated Ophiocordyceps sinensis Alleviates Diabetic Nephropathy and Its Podocyte Injury via Inhibiting P2X7R Expression and NLRP3 Inflammasome Activation

Table 2

Primer sequences for PCR analysis in cellular experiments.

Target	Primer sequence (5–3)	Length (bp)
Nephrin	Forward GTCTGGGGACCCCTCTATGA	209
	Reverse CAGGTCTTCTCCAAGGCTGT
Podocin	Forward CAGAAGGGGAAAAGGCTGCT	200
	Reverse GATGCTCCCTTGTGCTCTGT
WT-1	Forward TCCGGTCAGCATCTGAAACC	179
	Reverse GAGCTGGTCTGAGCGAGAAA
Desmin	Forward GTTTCAGACTTGACTCAGGCAG	106
	Reverse TCTCGCAGGTGTAGGACTGG
P2X7R	Forward CACCGTGCTTACAGGTGCTA	115
	Reverse CGGTCTTGGGGAACTCCTTC
NLRP3	Forward TCTGCACCCGGACTGTAAAC	131
	Reverse CATTGTTGCCCAGGTTCAGC
ASC	Forward GACAGTGCAACTGCGAGAAG	106
	Reverse CGACTCCAGATAGTAGCTGACAA
Procaspase-1	Forward ACAAGGCACGGGACCTATG	237
	Reverse TCCCAGTCAGTCCTGGAAATG
IL-1β	Forward CGCAGCAGCACATCAACAAG	118
	Reverse GTGCTCATGTCCTCATCCTG
IL-18	Forward ACTTTGGCCGACTTCACTGT	135
	Reverse GTCTGGTCTGGGGTTCACTG
GAPDH	Forward TGTGAACGGATTTGGCCGTA	202
	Reverse GATGGGCTTCCCGTTGATGA