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Journal of Diabetes Research
Volume 2018, Article ID 1614683, 7 pages
https://doi.org/10.1155/2018/1614683
Research Article

Association of PD-1 and PD-L1 Genetic Polymorphyisms with Type 1 Diabetes Susceptibility

1Department of Pharmacy, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
2Department of Endocrinology, The Second Affiliated Hospital of Soochow University, Suzhou 215004, China
3Department of Endocrinology, Jiangsu Province Hospital of TCM, 155 Hanzhonglu, Jiangsu Nanjing 210029, China

Correspondence should be addressed to Jie Pan; moc.361@napyknap and Chen Fang; moc.anis@1199afa

Received 11 April 2018; Accepted 15 October 2018; Published 11 November 2018

Academic Editor: Marco Songini

Copyright © 2018 Chenyue Qian et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Aims. The programmed death- (PD-) 1/PD-1 ligand (PD-L) pathway plays an important role in regulating T cell activation and maintaining peripheral tolerance. Accumulated studies showed that PD-1/PD-L1 pathway was involved in the development of type 1 diabetes (T1DM). Since the genetic background of type 1 diabetes differs greatly among the different population, we aim to investigate the association of genetic polymorphisms in PD-1 and PD-L1 with T1DM susceptibility in Chinese population. Methods. In total, 166 T1DM patients and 100 healthy controls were enrolled into the study. Genomic DNA was extracted from 4 mL peripheral blood samples collected from each subject. Genotyping of 8 selected SNPs of PD-1 and PD-L1 was carried out by the pyrosequencing PSQ 24 System using PyroMark Gold reagents (QIAGEN). Results. SNP rs4143815 in PD-L1 was significantly associated with T1DM. People carrying the C allele of rs4143815 suffering less risk of T1DM and T1DM patients with G/G genotype showed higher levels of autoantibody (AAB) positive incidence compared with C allele carriers. No significant associations were found in other SNPs. Conclusions. Our results indicate that rs4143815 of PD-L1 is significantly associated with T1DM and may serve as a new biomarker to predict the T1DM susceptibility.