Advanced Glycation End Products Increase MDM2 Expression via Transcription Factor KLF5

Figure 5

Evidence that KLF5 transcribed MDM2 in vitro and in vivo in a diabetic animal model. (a) Chromatin immunoprecipitation pulled down the first piece of KLF5-binding sequence (predicted 138 bp) in the human MDM2 gene. (b) Chromatin immunoprecipitation pulled down the third piece of the KLF5-binding sequence (predicted 166 bp) in the human MDM2 gene. (c) Chromatin immunoprecipitation pulled down the third and fourth KLF5-binding sequences in the mouse MDM2 gene, which was predicted to be 190 bp in size; H: healthy; D: diabetic. M: DNA size marker. Every experiment was repeated at least three times.

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