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Journal of Diabetes Research
Volume 2018, Article ID 3276162, 11 pages
Research Article

Evaluation of Aldose Reductase, Protein Glycation, and Antioxidant Inhibitory Activities of Bioactive Flavonoids in Matricaria recutita L. and Their Structure-Activity Relationship

1Department of Food Science and Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon, Republic of Korea
2College of Public Health, Hebei University, Baoding 071002, China
3Institute of Korean Nutrition, Hallym University, 1 Hallymdeahak-gil, Chuncheon, Republic of Korea
4Institute of Natural Medicine, Hallym University, 1 Hallymdeahak-gil, Chuncheon, Republic of Korea
5Hallym University Kangnam Sacred Heart Hospital, 1 Singil-ro, Yeoungdeungpo-gu, Seoul, Republic of Korea

Correspondence should be addressed to Jae Myung Yu; moc.liamtoh@uygnuymeaj

Received 13 November 2017; Accepted 4 January 2018; Published 10 April 2018

Academic Editor: Hiroshi Okamoto

Copyright © 2018 Seung Hwan Hwang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


The inhibitory activities of Matricaria recutita L. 70% methanol extract were evaluated by isolating and testing 10 of its compounds on rat lens aldose reductase (RLAR), advanced glycation end products (AGEs), and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging. Among these compounds, apigenin-7-O-β-D-glucoside, luteolin-7-O-β-D-glucoside, apigenin-7-O-β-D-glucuronide, luteolin-7-O-β-D-glucuronide, 3,5-O-di-caffeoylquinic acid, apigenin, and luteolin showed potent inhibition, and their IC50 values in RLAR were 4.25, 1.12, 1.16, 0.85, 0.72, 1.72, and 1.42 μM, respectively. Furthermore, these compounds suppressed sorbitol accumulation in rat lens under high-glucose conditions, demonstrating their potential to prevent sorbitol accumulation ex vivo. Notably, luteolin-7-O-β-D-glucuronide and luteolin showed antioxidative as well as AGE-inhibitory activities (IC50 values of these compounds in AGEs were 3.39 and 6.01 μM). These results suggest that the M. recutita extract and its constituents may be promising agents for use in the prevention or treatment of diabetic complications.