Evaluation of Aldose Reductase, Protein Glycation, and Antioxidant Inhibitory Activities of Bioactive Flavonoids in Matricaria recutita L. and Their Structure-Activity Relationship

Table 3

Inhibitory effects of rat lens aldose reductase-active compounds of Matricaria recutita L. on sorbitol accumulation in rat lens.

Compounds	Sorbitol content (mg)/lens wet weight (g)	Inhibition (%)
Sorbitol free by G free	No detection	—
Control by G	1.47 ± 0.02	—
Quercetin^1) by G + quercetin	0.21 ± 0.01^a	85.71 ± 3.23^a
Agipenin-7-O-β-D-glucoside (1)	0.72 ± 0.01^c	51.02 ± 1.68^c
Luteolin-7-O-β-D-glucoside (2)	0.07 ± 0.01^a	95.23 ± 8.18^a
Agipenin-7-O-β-D-glucuronide (5)	0.29 ± 0.01^b	80.27 ± 2.78^b
Luteolin-7-O-β-D-glucuronide (6)	0.12 ± 0.01^a	91.83 ± 6.74^a
3,5-O-di-caffeoylquinic acid (7)	0.20 ± 0.01^a	86.39 ± 4.28^a
Apigenin (9)	0.19 ± 0.01^a	87.07 ± 4.48^a
Luteolin (10)	0.12 ± 0.01^a	91.83 ± 6.87^a

¹⁾Quercetin was used as the positive control. Results are presented as (). Values within a column marked with different letters are significantly different from each other (). Samples concentration was used at 5 μg/mL on sorbitol accumulation in rat lens.