Early Growth Response 1 (Egr1) Is a Transcriptional Activator of NOX4 in Oxidative Stress of Diabetic Kidney Disease

Figure 1

Egr1, NOX4, ROS, and α-SMA expression in control and HFD/STZ-induced diabetic mice at 12 weeks. (a) Levels of Egr1, NOX4, and α-SMA mRNA were measured using RT-qPCR. (b) Levels of Egr1, NOX4, and α-SMA protein were measured using Western blotting assays. (c) Semiquantitative levels of Egr1, NOX4, and α-SMA protein. (d) NOX4 concentration, (e) ROS concentration, and (f) α-SMA concentration were measured using ELISAs. The results are expressed as foldchange over baseline (control group). Values are the mean ± SD. and versus the control group. (g) Masson staining and Immunohistochemical staining of Egr1, NOX4, and α-SMA.

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