MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy

Figure 3

MiR-22 upregulated was associated with reduced PTEN in DN in vivo and in vitro. (a) The expression of miR-22 in renal tissues of NC group and DN group was analyzed by qPCR; data represent mean ± SEM, , . (b) The protein level of PTEN in renal tissues of NC group and DN group was detected by Western blot (c) and analyzed by ImageJ; data represent mean ± SEM, , . (d) Representative photograph of immunohistochemical staining for PTEN protein expression analysis of renal tissues in NC group and DN group. Figures were shown at ×200 magnification. (e) NRK-52E cells were treated with NG or HG for 24 or 48 h. The expression of miR-22 was analyzed by qPCR; data represent mean ± SEM, , . (f) The expression of PTEN was detected by Western blot (g) and analyzed by ImageJ; data represent mean ± SEM, , .

(a)

(b)

(c)

(d)

(e)

(f)

(g)