Research Article

MicroRNA-22 Promotes Renal Tubulointerstitial Fibrosis by Targeting PTEN and Suppressing Autophagy in Diabetic Nephropathy

Figure 4

MiR-22 directly targets PTEN. (a) Predicted binding sequences and mutated site between miR-22 and seed site in 3UTR of PTEN. (b) NRK-52E cells were transfected with miR-22 mimics or NC mimics for 24 h. qPCR was performed to detect the transfection efficiency of miR-22 mimics. (c) NRK-52E cells were cotransfected with 50 nM miR-22 mimics or NC mimics, 100 ng of the WT or MUT plasmid that contain 3UTR of PTEN, and 5 ng Renilla plasmid; data represent mean ± SEM, , . (d) After transfected with miR-22 mimics or NC mimics for 24 h, NRK-52E cells were treated with NG or HG for another 48 h. The expression of PTEN was detected by Western blot.
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