Effects of Type 2 Diabetic Serum on Proliferation and Osteogenic Differentiation of Mesenchymal Stem Cells

Figure 3

Proliferation of MSCs cultured with serum containing different levels of HbA1c, detected with an MTT assay. MCSs were divided into four groups, to which were added human blood sera with different levels of HbA1c: <6.5%, 6.5%–8.0%, 8.0%–10%, and >10%. After 6 days in culture, the proliferation of the MSCs differed significantly. Diabetic serum containing 8%–10% HbA1c stimulated cell proliferation most effectively. All results were expressed as the and analyzed using one-way ANOVA. Student–Newman–Keuls multiple-range test was used to compare results between two groups: versus group above 10%, ; versus group 6.5–8.0%, , ; versus group below 6.5%, . .