Research Article

The Iron-Klotho-VDR Axis Is a Major Determinant of Proximal Convoluted Tubule Injury in Haptoglobin 2-2 Genotype Diabetic Nephropathy Patients and Mice

Figure 1

Iron staining in Hp 2-2 and Hp 1-1 renal sections of diabetic nephropathy and nondiabetic mice. (a) There was a significant increase of iron deposits represented by the DAB-enhanced Prussian blue staining (brown, indicated by a red arrow) in the proximal convoluted tubule (PCT) of Hp 2-2 diabetic (DM) sections compared with diabetic Hp 1-1 or with Hp 2-2 nondiabetic (nonDM) sections. (b) Quantification of the iron staining shows a significant increase in the percentage of the iron-stained area of Hp 2-2 DM mice compared with DN Hp 1-1 mice () or compared with nonDM Hp 2-2 mice ().
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