The Iron-Klotho-VDR Axis Is a Major Determinant of Proximal Convoluted Tubule Injury in Haptoglobin 2-2 Genotype Diabetic Nephropathy Patients and Mice

Figure 3

(a) α-Klotho renal expression in nondiabetic and diabetic nephropathy mice. (b) The percentage of the α-klotho-stained area is significantly decreased in the renal tubules of Hp 2-2 diabetic (DM) sections compared with Hp 1-1 diabetic () or with nondiabetic (nonDM) Hp 2-2 sections (). (c) α-Klotho expression in mice renal lysates from Hp 1-1 and Hp 2-2 nondiabetic (nonDM) and diabetic (DM) mice. Proteins were detected by immunoblotting with antibodies against α-klotho and actin. The values represent the mean ± SEM for 3 independent experiments subjected to 2-tailed, 2-sampled unequal variance Student’s t-test, with compared with Hp 2-2 nonDM and compared with Hp 1-1 DM. AU: arbitrary units.

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