TERT and Akt Are Involved in the Par-4-Dependent Apoptosis of Islet β Cells in Type 2 Diabetes

Figure 5

Diabetes activates Par-4 and inhibits p-Akt, inducing NIT-1 cell apoptosis. (a) Apoptosis was detected via TUNEL staining in the C, CS, H, and HS groups. (b) Western blot analysis of Par-4, Akt, and p-Akt expression; apoptosis rates; cell survival rates determined with MTT assays; and glucose-stimulated insulin secretion in the C, CS, H, and HS groups. (c) Apoptosis was detected via TUNEL staining in the CS, HS, CS-Par-4, and HS-Par-4 groups. (d) Western blot analysis of Par-4, Akt and p-Akt expression, apoptosis rates, cell survival rates determined with MTT assays, and glucose-stimulated insulin secretion in the CS, HS, CS-Par-4, and HS-Par-4 groups. Compared with the C group, . ^#Compared with the H group, . ^aCompared with the CS group, . ^bCompared with the HS group, .

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