Contractile Protein Expression and Phosphorylation and Contractility of Gastric Smooth Muscles from Obese Patients and Patients with Obesity and Diabetes

Figure 3

MYPT1 expression and T853 phosphorylation in nondiabetic and diabetic gastric fundus and gastric antrum smooth muscles. (a) Representative immunoblots of MYPT1 expression in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group), 1 μg muscle lysate per lane. (b) MYPT1 expression relative to enteric γ-actin expression in nondiabetic and diabetic fundus and antrum smooth muscles. (c) Representative immunoblots of phosphorylated T853 in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group), 1 μg muscle lysate per lane. (d) T853 phosphorylation relative to MYPT1 expression in nondiabetic and diabetic fundus and antrum smooth muscles. (e) Representative immunoblots of phosphorylated T696 in nondiabetic and diabetic fundus (left panel, , each group) and nondiabetic and diabetic antrum muscles (right panel, , each group), 1 μg muscle lysate per lane. (f) T696 phosphorylation relative to MYPT1 expression in nondiabetic and diabetic fundus and antrum smooth muscles. , , and .

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