Proteomic Analysis of Hippocampus and Cortex in Streptozotocin-Induced Diabetic Model Mice Showing Dementia

Table 3

Identified phosphoproteins in the hippocampus and cortex, and their functional categories.

Spot no.	Accession no.	Identified protein	Observed Mw (kDa)/pI	Theoretical Mw (Da)/pI	Score	SC	Fold change (STZ/CTL)	ANOVA value
Hippocampus
Neuronal development
35	gi|40254595	Dihydropyrimidinase-related protein 2	44/5.6	62,638/5.95	85	4	1.2	0.04
36	gi|40254595	Dihydropyrimidinase-related protein 2	44/5.8	62,638/5.95	144	6	1.1	0.015
Protein degradation
37	gi|261824000	Proteasome subunit alpha type-3	28/5.3	28,615/5.29	88	9	0.9	0.037
Vesicular transport
38	gi|29789104	Beta-soluble NSF attachment protein	30/5.3	33,878/5.32	177	16	0.8	0.04
Cortex
Cytoskeletal protein
42	gi|809561	Gamma-actin	43/5.3	41,335/5.56	254	13	0.7	0.039
Neuronal development
39	gi|40254595	Dihydropyrimidinase-related protein 2	45/5.9	62,638/5.95	490	17	1.3	0.026
Protein degradation
41	gi|261824000	Proteasome subunit alpha type-3	21/5.3	28,615/5.29	142	14	0.8	0.043
Unknown
40	gi|70794816	Enolase 1B, retrotransposed	32/6.5	47,453/6.37	163	9	1.1	0.018

Spot numbers correspond to the 2-DE gel in Figure 2. Accession numbers were obtained from the National Center for Biotechnology Information. (NCBI) database. Score and sequence coverage (SC) were obtained by Mascot database searching (http://www.matrixscience.com). values were obtained by ANOVA, .