Research Article
Spironolactone Protects against Diabetic Cardiomyopathy in Streptozotocin-Induced Diabetic Rats
Table 1
Sequences of primers used for real-time PCR.
| | Gene access number | Forward | Reverse |
| | Sirt-1 | 309757 | 5-TGATTGGCACCGATCCTCG-3 | 5-TGGATGCTCTCATCAGGACAG-3 | | PGC-1α | 83516 | 5-AAGTGGTGTAGCGACCAATCG-3 | 5-AATGAGGGCAATCCGTCTTCA-3 | | ATP5α1 | 65262 | 5-GAGACTGGGCGTGTGTTAAG-3 | 5-CTCCTCTGCTTGAACATTCCTC-3 | | COX-5b | 94194 | 5-ACCCTAATCTAGTCCCGTCC-3 | 5-CAGCCAAAACCAGATGACAG-3 | | TGF-β1 | 59086 | 5-CCTGCAAGACCATCGACATG-3 | 5-TGTTGTACAAAGCGAGCACC-3 | | Collagen 1 | 29393 | 5-TCAAGATGGTGGCCGTTACT-3 | 5-CATCTTGAGGTCACGGCATG-3 | | TNF-α | 24835 | 5-TCATCCGTTCTCTACCCAGC-3 | 5-TACTTCAGCGTCTCGTGTGT-3 | | MCP-1 | 24770 | 5-ACCAGCCAACTCTCACTGAA-3 | 5-GCCAGTGAATGAGTAGCAGC-3 | | Nrf-1 | 312195 | 5-GAGTGACCCAAACCGAACA-3 | 5-GGAGTTGAGTATGTCCGAGT-3 | | GCLC | 25283 | 5-GATGATAGAACACGGGAGG-3 | 5-CATTGGTCGGAACTCTACTC-3 | | Nox4 | 85431 | 5-TGTCTGCATGGTGGTGGTAT-3 | 5-CTTCAACAAGCCACCCGAAA-3 | | GAPDH | 24383 | 5-CTGGAGAAACCTGCCAAGTATGAT-3 | 5-TTCTTACTCCTTGGAGGCCATGTA-3 |
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Sirt-1: NAD-dependent deacetylase sirtuin-1; PGC-1α: peroxisome proliferator-activated receptor gamma coactivation factor 1α; ATP5α1: ATP synthase 5a; COX-5b: cytochrome oxidase 5b; TGF-β: transforming growth factor β; TNF-α: tumor necrosis factor α; MCP-1: monocyte chemoattractant protein 1; Nrf-1: nuclear respiratory factor 1; GCLC: glutamate cysteine ligase catalytic subunit; Nox4: NADPH oxidase 4; GAPDH: glyceraldehyde 3-phosphate dehydrogenase.
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