Novel Mechanisms Modulating Palmitate-Induced Inflammatory Factors in Hypertrophied 3T3-L1 Adipocytes by AMPK

Figure 5

Effects of metformin on palmitate-induced MCP-1 protein and intracellular TG contents in 24 h palmitate-preloaded 3T3-L1 adipocytes. Differentiated 3T3-L1 adipocytes were exposed to 0.3 mmol/L palmitate (black bar) or ethanol vehicle alone (white bar) in the presence or absence of 2.5 mmol/L metformin for 24 h. Lysates were immunoblotted to assess the phosphorylation of AMPK on Thr172 (a) and intracellular MCP-1 (b). The cellular TG (c) contents were measured and then adjusted to intracellular total protein contents. Data are means ± SEM (). , compared to corresponding control cells.

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