Food Intake and Core Body Temperature of Pups and Adults in a db Mouse Line Deficient in the Long Form of the Leptin Receptor without Misty Mutation

Table 2

Organ weight and biochemical parameters including hormones at 2 and 8 weeks.

		2 weeks		8 weeks		values (two-way ANOVA)
Male		WT ()	db/db ()	WT ()	db/db ()	Age	Genotype	Interaction
Body weight	g	6.15 ± 0.18^a	6.41 ± 0.21^a	24.91 ± 0.30^b	41.82 ± 0.75^c	(<0.01)	(<0.01)	<0.01
Milk intake^#	g/h	0.146 ± 0.015	0.153 ± 0.024^NS
Food intake	g/day			3.42 ± 0.09	7.60 ± 0.33
Organs
Brain	mg	355.0 ± 5.6^ab	335.5 ± 5.9^a	421.7 ± 3.6^c	363.3 ± 5.1^b	(<0.01)	(<0.01)	<0.01
Heart	mg	34.8 ± 2.0	30.0 ± 1.1	120.9 ± 1.5	117.9 ± 2.8	<0.01	0.055	0.660
Liver	g	0.19 ± 0.01^a	0.19 ± 0.01^a	1.36 ± 0.03^b	2.79 ± 0.11^c	(<0.01)	(<0.01)	<0.01
WAT	g	0.013 ± 0.002^a	0.026 ± 0.003^a	0.250 ± 0.013^b	1.781 ± 0.073^c	(<0.01)	(<0.01)	<0.01
BAT	mg	61.0 ± 6.5	76.5 ± 6.3	103.2 ± 8.0	198.9 ± 49.4	<0.01	0.037	0.126
Plasma
Glucose	g/l	1.12 ± 0.05^a	1.01 ± 0.04^a	2.29 ± 0.19^a	6.14 ± 0.89^b	(<0.01)	(<0.01)	<0.01
TG	g/l	0.68 ± 0.10	0.79 ± 0.12	1.04 ± 0.13	1.20 ± 0.17	<0.01	0.284	0.844
Insulin	ng/ml	0.40 ± 0.03^a	0.59 ± 0.08^a	0.99 ± 0.09^a	9.61 ± 3.53^b	(<0.01)	(<0.019)	0.024
Leptin	ng/ml	4.0 ± 1.1^a	46.6 ± 5.4^b	1.1 ± 0.3^a	94.6 ± 7.2^c	(<0.01)	(<0.01)	<0.01
Corticosterone	ng/ml	43.6 ± 7.1^a	56.3 ± 8.7^a	148.0 ± 33.2^a	292.3 ± 52.1^b	(<0.01)	(<0.013)	0.034
T3	ng/ml	2.80 ± 0.01	2.92 ± 0.14	1.16 ± 0.03	1.22 ± 0.04	<0.01	0.235	0.688
T4	ng/ml	98.6 ± 3.5	81.5 ± 5.2	41.6 ± 1.5	33.7 ± 0.9	<0.01	<0.01	0.172
TSH	μIU/ml	6.71 ± 0.47	7.06 ± 0.40	0.99 ± 0.23	1.85 ± 0.75	<0.01	0.236	0.617
Female		WT ()	db/db ()	WT ()	db/db ()	Age	Genotype	Interaction
Body weight	g	6.01 ± 0.11^a	6.37 ± 0.14^a	19.38 ± 0.21^b	39.27 ± 0.92^c	(<0.01)	(<0.01)	<0.01
Milk intake	g/h	0.093 ± 0.024	0.102 ± 0.031^NS
Food intake	g/day			3.03 ± 0.09	7.34 ± 0.43^∗∗

WT: wild type (homozygous wild type in leptin receptor gene); db/db: leptin receptor deficiency (homozygous mutants in leptin receptor gene); BW: body weight; WAT: white adipose tissue; BAT: brown adipose tissue; TG: triglyceride; T3: triiodothyronine; T4: thyroxine; TSH: thyroid-stimulating hormone. ^#The number of male samples for milk intake measurement was 7. All data were obtained from mice after 3 h fasting except milk and food intake (described in detail in Materials and Methods). Values are the mean ± SE. Values except milk and food intake were analyzed by two-way ANOVA (interaction, age, and genotype) followed by the Tukey-Kramer method when significant. Bold values mean the significance in the interaction. In the case, the main effect of age and genotype is shown by the parenthesis as a reference. Values of milk and food intake were analyzed by unpaired Student’s t-test, respectively. The same superscript letter and NS indicate that the difference was not significant. vs. WT mice at the respective age.