Research Article

Effect of Baoshenfang Formula on Podocyte Injury via Inhibiting the NOX-4/ROS/p38 Pathway in Diabetic Nephropathy

Figure 5

Effect of BSF on NOX-4-mediated oxidative stress in cultured podocyte. (a) Representative band of NOX-4 protein in cultured podocyte. (b) Comparison of the grey values of NOX-4 protein in cultured podocyte. (c) Comparison of mRNA levels of NOX-4 in cultured podocyte. BSF suppressed the upexpression of NOX-4 after exposure of podocyte to high glucose (HG). (d–f) Comparison of MDA, NOS, and T-SOD levels measured by ELISA in cultured podocyte. (g, h) Comparison of ROS production in cultured podocyte. High glucose induced a marked enhancement of MDA, NOS, and ROS levels, a significant suppression of T-SOD level. Compared with the HG group, MDA, NOS, and ROS levels were significantly decreased, while the T-SOD level was significantly increased in the BSF group. And the upregulated ROS production was suppressed by NOX-4 silencing. , versus normal control (NC), , and versus HG.