Research Article

Combined Administration of Streptozotocin and Sucrose Accelerates the Appearance of Type 2 Diabetes Symptoms in Rats

Table 4

Glucose tolerance test (GTT) and insulin tolerance test (ITT) of rats of experimental protocol B. The data shown are of the Control (males , females ), C+10% 7w (males , females ), C+10% 11w (males , females ), C+30% 7w (males , females ), C+30% 11w (males , females ), Stz70 (males , females ), Stz70+10% 7w (males , females ), Stz70+10% 11w (males , females ), Stz70+30% 7w (males , females ), and Stz70+30% 11w (males , females ) groups. Data are represented in percentages, considering the initial glucose value as 100%. Values (except initial glucose) are expressed as the median and interquartile range (IQR) (p25-p75). For the GTT, the rats were given glucose (2 g/kg body weight) orally, and for the ITT, they were injected with insulin (0.5 IU/kg body weight) i.p. Blood glucose level was monitored at 30, 60, and 120 min after the administration of glucose or insulin. The area under the curve (AUC) was calculated using the trapezoidal rule in GraphPad Prism. Variation between more than two groups was measured by the Kruskal-Wallis test followed by the Dunn Multiple Comparison Test. The asterisk denotes that the data are significantly different from the Control group (, , and ), and the plus sign denotes that the data are significantly different from the Stz70 group (+, ++, and +++).

GTTITT
0 min30 min60 min120 minAUC0 min30 min60 min120 minAUC

MaleControl100147.5 (116.1-180.6)133.1 (129.7-156)114.3 (97.46-133)505.4 (459.4-575.2)10055.4 (51.08-63.73)68.8 (48.96-74.9)80 (58.6-89.3)288.7 (247.2-303.2)
C+10% 7w100151.3 (118.5-167.7)137.7 (104.6-178.6)98.8 (93.86-119.5)532.3 (429.2-569.4)10082.9 (62.6-87.8)62.22 (55.1-88.2)80.18 (76.8-123.5)334 (274.2-377.8)
C+10% 11w100144.3 (123.8-154.1)131.2 (105.4-146.2)104.9 (77.34-129.6)474.4 (429.4-543.6)10069.5 (63.2-73)79.1 (71.7-88.3)95.2 (91-104.3)341.4(320.8-346.7)
C+30% 7w100202.4 (165.5-235.5)175.4 (149.7-202.9)153.9 (126.4-177.2)650.4 (573-775.4)10085.91 (69.4-100.3)92.94 (65-97.6)100 (87.1-117)375.4(298.2-426.8)
C+30% 11w100176.2 (146.1-203)143.4 (132.4-168)129.5 (115.8-156.3)596.8 (521.8-640.8)10072.9 (60.3-84.3)76.6 (50.5-92.6)104.8 (81.3-132.3)343.5 (290-378.1)
Stz70100236 (190.9-279.4)216.7 (195.5-247.7)184.1 (151.3-192.2)796.2 (689.5-860.8)10063.57 (61.8-89.8)58.02 (37.1-66.7)76.58 (48.2-98.8)291.4 (268.2-369.1)
Stz70+10% 7w100205.3 (162.2-232.5)205.7 (159.3-230.1)158.2 (114.8-184.9)722.1 (568.9-809.7)10086.49 (58.3-115.2)55.56 (24.1-76)49.69 (23-89.5)289.3 (165.4-364.1)
Stz70+10% 11w100209.8 (145.5-280.9)202.7 (142.3-219.6)156.3 (108.8-188.8)737.8 (526.7-822.4)10072.58 (48.9-91.8)56.56 (33.4-81.1)72.59 (41.3-88.8)291.5 (191.2-339.8)
Stz70+30% 7w100282.2 (172.1-303.8)278.3 (172.4-322.3)228.3 (143.1-242)984.6 (633-1064)10065.09 (52.6-69.2)40.57 (37.4-78.1)40.54 (34.6-66.8)215.4 (193.8-314.7)
Stz70+30% 11w100242.7 (208.9-270.8)254.5 (160.7-291.4)202.3 (126.9-224.4)876.8 (626.9-982.4)10055.9+ (52.7-65)37.3 (34.6-55.4)57.8 (51.4-92.3)229.8 (208.3-283)

FemaleControl100174.7 (143.9-196.4)149 (125.3-160.5)113.3 (101.5-149.7)547.5 (484.1-632.7)10054.4 (52.3-60.2)64.2 (48.5-75)87.5 (72.7-101.1)315.9 (258.2-352)
C+10% 7w100151 (125.6-171.3)142.3 (122.6-156.7)107.6 (90.11-138.9)513.8 (487.6-565.6)10057.58 (55.4-59.6)52.08 (49.9-54.4)81.3 (67-93.5)265.6 (258.1-275.2)
C+10% 11w100169 (150-216.3)132.1 (120.4-152.1)113.1 (101.7-142.9)509 (492.3-637.3)10060.8 (52.2-67.2)59.45 (42.5-72)86.6 (73.6-110)286.8 (243.6-330.7)
C+30% 7w100145.2 (125.7-166.6)161.8 (128.6-200.7)113.8 (103.2-133.1)563.9 (417.7-638.6)10056.76 (51.9-67.8)47.8 (39.7-61.5)64.1 (56.3-82.7)248.4 (217.7-286.9)
C+30% 11w100217 (125.7-236.5)149 (116.9-177.5)126.4 (102.9-158.6)631.4 (427.7-708.5)10057.83 (51.9-90.5)62.3 (56.1-87.8)101.1 (85.2-161.9)301.9 (263.3-437.4)
Stz70100253.7 (205.7-286.5)243.4 (227.8-277.2)158.3 (118.4-220.6)822.4 (803.7-926.2)10073.3 (56.9-123.5)75.2 (49.6-121.7)110.5 (79.8-142.9)318.8 (230.6-342.9)
Stz70+10% 7w100198 (178-272.4)175.6 (167.5-189.7)82.7+ (71.03-90.24)630 (582-677.9)10037.6++ (34.7-40.4)32.8+ (30.1-35.6)47.6+ (47-48.2)184.4++ (182.5-186.2)
Stz70+10% 11w100225.7 (225.7-282.4)196.1 (196.1-243.4)138.3+ (125-138.3)708.3 (693-725.2)10063.53 (60.2-66.9)49.63 (49.6-49.6)82.05 (79.1-85)270.5 (221.3-299)
Stz70+30% 7w100260.5 (251.1-275.4)259.1 (219.5-284)145.5 (108.7-182.9)859 (752.5-903.7)10045.14 (39.5-65.5)41.08 (31.1-55.7)49.69 (43.7-80.5)199.6++ (185.9-286.4)
Stz70+30% 11w100252.5 (205.7-282.9)286.6 (268.8-329.7)238.2 (220.6-253.8)1081 (994-1245)10051.34 (41.9-60.8)59.95 (56.2-63.7)86.06 (85-87.2)277.4 (274.6-280.1)